Variabilidad genética de Loci microsatélites en los primeros lotes de Litopenaeus vannamei introducidos en Cuba para la acuicultura

  1. Y.J. Borrell 12
  2. G. Espinosa 1
  3. E. Vázquez 2
  4. J.A. Sánchez 2
  5. G. Blanco 2
  1. 1 Universidad de La Habana
    info

    Universidad de La Habana

    La Habana, Cuba

    ROR https://ror.org/04204gr61

  2. 2 Universidad de Oviedo
    info

    Universidad de Oviedo

    Oviedo, España

    ROR https://ror.org/006gksa02

Aldizkaria:
Revista de investigaciones marinas

ISSN: 1991-6086,  0252-1962

Argitalpen urtea: 2006

Alea: 27

Zenbakia: 3

Orrialdeak: 237-244

Mota: Artikulua

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Beste argitalpen batzuk: Revista de investigaciones marinas

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Four microsatellite loci have been used in this work to characterize the first two stocks of Litopenaeus vannameiintroduced in Cuba for aquaculture; the possibility of establish an efficient broodstock it is also evaluated. Our results show levels of genetic variation (mean heterozygosity Ho=0,560, mean number of alleles Na=7,5) lower than other previously reported for wild and cultured populations in this specie. Relatedness coefficients show that at least in one of the stock under analysis exist high degrees of kinship among individuals (Stock 1 mean r=-0.0080, Stock 2 mean r=+0.1515). It is advisable to collect all the possible genetic variation that exists in the source wild shrimp population or even from other broodstocks genetically different although sharing the same origin in order to establish the first broodstock. We propose a strategy that includes gametes crio-conservation and the use of microsatellite to avoid endogamy and calculate heritability of economically interesting traits.

Erreferentzia bibliografikoak

  • Allendorf FW y N. Ryman (1987): Genetic management of hatchery stocks. In: Population Genetics and Fishery Management (N. Ryman y F. Utter, eds.), Seattle: University of Washington Press, pp : 141–159.
  • Anónimo (1993): China's shrimp crop failure may cause supply-demand imbalance. Asian. Shrimp News, 16.
  • Bangkok (2000): Aquaculture Development Beyond 2000: The Bangkok Declaration and Strategy. Bangkok. Thailandia, 21 pp.
  • Bartley, D.M. (1998): Genetics and breeding in aquaculture: current status and trends. In: Genetics and Breeding of Mediterranean Aquaculture Species (D.M. Bartley y B. Basurco, eds), Cahiers Options Vol. 34, pp : 13- 30.
  • Benzie, J.A.H. (1996): Penaeid Genetics and Biotechnology. Second International Conference on the Culture of Penaeid Prawns and Shrimps, Iloilo City, Philippines.
  • Benzie, J.A.H. (2000): Population genetic structure in penaeid prawns. Aquaculture Research 31:95– 119.
  • Bierne, N., I. Beuzart, V. Vonau, F. Bonhomme y E. Bedier (2000): Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris. Aquaculture 184:203–219.
  • Borrell, Y.J, E. Vázquez, J.A. Sánchez y G. Blanco (2002): Esclarecimiento de relaciones de parentesco en Salmo salar L., 1578 utilizando loci microsatélites. Boletín del Instituto Español de Oceanografía 18(1-4):211-220.
  • Borrell, Y.J., J. Álvarez, E. Vázquez, C. Fernández, C. Martínez, J.A. Sánchez y G. Blanco (2004): Applying microsatellites to the management of turbot stocks (Scophthalmus maximus L.) in hatcheries. Aquaculture 241:133-150.
  • Cruz, P., C.H. Mejia-Ruíz, R. Pérez-Enríquez y A.M. Ibarra (2002): Isolation and characterization of microsatellites in Pacific white shrimp Penaeus (Litopenaeus) vannamei. Molecular Ecology Notes 2:239-241.
  • Cruz, P., A.M. Ibarra, H. Mejía-Ruíz, P.M. Gaffney y R. Pérez-Enríquez (2004): Genetic variability assessed by microsatellites in a breeding program of pacific white shrimp Litopenaeus vannamei. Marine Biotechnology 6:157-164.
  • Danzmann, R.G., T.R. Jackson, M.M. Ferguson y P.E. Ihssen (1997): The identification of multiple QTL influencing temperature tolerance in rainbow trout and their phenotypic effects and genomic backgrounds. Sixth International Symposium on Genetics and Aquaculture, Sttirling, Scotland.
  • Dib, C., S. Faure, C. Fizames, D. Samson, N. Drouot, A. Vignal y P. Millasseau (1996) : A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature 380: 152-154.
  • Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Bolesy Z. Husain (1996): A comprehensive genetic map of the mouse genome. Nature 380:149- 152.
  • Fenster, C.B. y L.F. Galloway (2000): Inbreeding and outbreeding depression in natural populations of Chamaecrista fasciculata (Fabaceae). Conservation Biology 14 (5):1406-1412.
  • García, D.K., M.A. Faggart, L. Rhoades, A. Alcivar Warren, J.A. Wyban, W.H. Carr y J.N. Sweeney (1994): Genetic diversity of cultured Penaeus vannamei shrimp using three molecular genetic techniques. Molecular Marine Biology and Biotechnology 3:270–280.
  • Gjerde, B., K. Gunnes y T. Gjedrem (1983): Effect of inbreeding on survival and growth in rainbow trout. Aquaculture 34: 327– 332.
  • Goudet, J. (1995): FSTAT (vers. 1.2): a computer program to calculate F-statistics. Journal of Heredity 86:485–486.
  • Goudet, J. (2001): FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). http://www.unil.ch/izea/softwares/fstat. tml
  • Herbinger, C.M., P.T. O’Reilly, R.W. Doyle, J.M. Wright y F. O’Flynn (1999) : Early growth performance of Atlantic salmon full-sib families reared in single family tanks versus in mixed family tanks. Aquaculture 173 (1–4):105– 116.
  • Jackson, T.M., D.J. Martín-Robichaud y M.E. Reith (2003): Application of DNA markers to the management of Atlantic halibut (Hippoglossus hippoglossus) broodstock. Aquaculture 220: 245–259.
  • Jackson, T.R., M. Ferguson, R. Danzmann, A. Fishback, P. Ihssen, M. O´Connell y T. Crease (1998): Identification of two QTL influencing upper temperature tolerance in three rainbow trout (Oncorhynchus mykiss) half-sib families. Heredity 80:143-151.
  • Keller, M., J. Kollmann y P.J. Edwards (2000): Genetic introgression from distant provenances reduces fitness in local weed populations. Journal of Applied Ecology 37:647-659.
  • Kincaid, H.L. (1983): Inbreeding in fish populations used for aquaculture. Aquaculture 33, 215–227.
  • Knibb, W. (2000): Genetic improvement of marine fish-which method for industry? Aquaculture Research 31: 11-23.
  • Lie, O., R.G. Danzmann, R. Guyomard, L.E. Holm, B. Hoyheim, R. Powell, A. Slettan y J. Taggart (1997): Constructing a genetic map of salmonid fishes: SALMAP. Sixth International Symposium on genetics and Aquaculture, Sttirling, Scotland.
  • McGoldrick, D.J., D. Hedgecock, L. English, P. Baoprasertkul y R.D. Ward (2000): The transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas): selection and null alleles. Journal of Shellfish Research 19 (2): 779– 788.
  • Nei, M. (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590.
  • Nei, M. (1987): Molecular Evolutionary Genetics. Columbia University Press, New York, 432 pp.
  • Norris, A.T., D.G. Bradley y E.P. Cunningham (2000): Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture 182: 73– 83.
  • O’ Connell, M. y J.M. Wright (1997): Microsatellite DNA in fishes. Rev. Fish Biol. Fish. 7: 331-363.
  • Pemberton, J.M., J. Slate, D.R. Bancroft y J.A. Barrett (1995): Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Molecular Ecology 4:249–252.
  • Primavera, J.H. (1998): Tropical shrimp farming and its sustainability. In: Tropical Mariculture (S.S. De Silva, eds), Academic Press, London, UK, 487pp.
  • Queller, D.C. y K.F. Goodnight (1989): Estimating relatedness using genetic markers. Evolution 43: 258– 275.
  • Rice, W.R. (1989): Analysing tables of statistical tests. Evolution 43:223–225.
  • Sbordoni, V., M. de Matthaeis, M. Cobolli Sbordoni, G. La Rosa y M. Mattoccia (1986): Bottleneck effects and the depression of genetic variability in hatchery stocks of Penaeus japonicus (Crustacea, Decapoda). Aquaculture 57:239-251.
  • Su, G.S., L.E. Liljedabl y G.A.E. Gall (1996): Effects of inbreeding on growth and reproductive traits in rainbow trout (Oncorhynchus mykiss). Aquaculture 142: 139–148.
  • Swofford, D.L. y R.B. Selander (1981): Biosys-1: a FORTRAN program for the comprehensive analysis for electrophoretic data in population genetics and systematics. Journal of Heredity 72:281–283.
  • Walsh, P.S., D.A. Metzger y R. Higuchi (1991): Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506–510.
  • Wolfus, G.M., G.K. García y A. Alcivar-Warren (1997): Application of the microsatellites technique for analyzing genetic diversity in shrimp breeding programs. Aquaculture 152:35–47.
  • Xu, Z., J.H. Primavera, L.D. de la Pena, P. Pettit, J. Belak y A. Alcivar-Warren (2001): Genetic diversity of wild and cultured black tiger shrimp (Penaeus monodon) in the Philippines using microsatellites. Aquaculture 199:13–40.
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