Antagonistic Action of Yucasine and DMSO on Apogamy in the Fern Dryopteris affinis ssp. affinis
- Sánchez, Eugenio
- Menéndez, Alejandro
- Rivera, Alejandro
- Cañal, María Jesús
- Fernández, Helena
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1
Universidad de Oviedo
info
ISBN: 9789811661693, 9789811661709
Año de publicación: 2022
Páginas: 309-323
Tipo: Capítulo de Libro
Resumen
Apogamy is a peculiar case of apomixis, very frequent in ferns, in which an asexual embryo evolves from somatic cells of the gametophyte generation. This work reports about the effect of the yucasine, an inhibitor of the auxin biosynthesis, on vegetative and reproductive development of the gametophyte of Dryopteris affinis ssp. affinis, an obligate apogamous species. To cope with it, homogenized gametophytes were cultured in vitro in liquid Murashige and Skoog medium, supplemented with sucrose 2% (w/v), and tested up to three concentrations of yucasine (0.47, 4.7, and 23.6 μM). This compound was dissolved using dimethyl sulfoxide (DMSO), and therefore a control with the maximum dose (0.5%) used of this agent was included in the experiment.
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