Stevia preferences in Wistar rats

  1. Paula Núñez Martínez 1
  2. Juan Argüelles Luis 1
  3. Carmen Perillán Méndez 1
  1. 1 Universidad de Oviedo (España)
Journal:
Psicothema

ISSN: 0214-9915

Year of publication: 2016

Volume: 28

Issue: 4

Pages: 442-447

Type: Article

More publications in: Psicothema

Abstract

Background: The Stevia rebaudiana plant is likely to become a major source of high-potency sweetener for the growing natural-food market. S. rebaudiana is the source of a number of sweet diterpenoid glycosides, but the major sweet constituents are rebaudioside A and stevioside. These two constituents have similar pharmacokinetic and metabolic profiles in rats and humans, and thus, studies carried out with either steviol glycoside are relevant to both. Other studies illustrate the diversity of voluntary sweet intake in mammals. Method: This study was done using a series of two-bottle tests that compared a wide range of sweetener concentrations versus saccharin concentrations and versus water. Results: Wistar rats displayed preferences for stevia extract and pure rebaudioside A solutions over water at a range of concentrations (0.001% to 0.3%), and their intake peak occurred at 0.1% concentration. They also preferred solutions prepared with a commercial rebaudioside A plus erythritol mixture to water, and their peak was at 2% concentration. Conclusions: The present study provides new information about the responses of Wistar rats to stevia compounds and commercial stevia products such as Truvia. These results could help with the appropriate dosage selection for focused behavioral and physiological studies on stevia.

Bibliographic References

  • Atchley, D. P., Weaver, K. L., & Eckel, L. A. (2005). Taste responses to dilute sucrose solutions are modulated by stage of the estrous cycle and fenfl uramine treatment in female rats. Physiology and Behavior, 86, 265-271.
  • Bachmanov, A. A., Bosak, N. P., Floriano, W. B., Inoue, M., Li, X., Lin, C., Murovets, V. O., Reed, D. R., Zolotarev, V. A., & Beauchamp, G. K. (2011). Genetics of sweet taste preferences. Flavour and Fragrance Journal, 26, 286-294.
  • Bachmanov, A. A., Bosak, N. P., Lin, C., Matsumoto, I., Ohmoto, M., Reed, D. R., & Nelson, T. M. (2014). Genetics of taste receptors. Current Pharmaceutical Design, 20, 2669-2683.
  • Bachmanov, A. A., Reed, D. R., Beauchamp, G. K., & Tordoff, M. G. (2002). Food intake, water intake, and drinking spout side preference of 28 mouse strains. Behavior Genetics, 32, 435-443.
  • Bachmanov, A. A., Tordoff, M. G., & Beauchamp, G. K. (2001). Sweetener preference of C57BL/6ByJ and 129P3/J mice. Chemical Senses, 26, 905-913.
  • Balcombe, J. P., Barnard, N. D., & Sandusky, C. (2004). Laboratory routines cause animal stress. Contemporary Topics in Laboratory Animal Science, 43, 42-51.
  • Barrett, A. M., & Stockham, M. A. (1963). The effect of housing conditions and simple experimental procedures upon the corticosterone level in the plasma of rats. Journal of Endocrinology, 26, 97-105.
  • Carakostas, M. C., Curry, L. L., Boileau, A. C., & Brusick, D. J. (2008). Overview: The history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverages. Food and Chemical Toxicology, 46, 1-10.
  • Collier, G., & Novell, K. (1967). Saccharin as a sugar surrogate. Journal of Comparative Physiology, 64, 401-408.
  • Debnath, M. (2008). Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. Journal of Medicinal Plants Research, 2, 45-51.
  • Dess, N. K., Chapman, C. D., & Monroe, D. (2009). Consumption of SC45647 and sucralose by rats selectively bred for high and low saccharin intake. Chemical Senses, 34, 211-220.
  • Fregly, M. J., & Rowland, N. E. (1992). Comparison of preference thresholds for NaCl solution in rats of the Sprague-Dawley and LongEvans strains. Physiology and Behavior, 51, 915-918.
  • Fujita, Y., Wideman, R. D., Speck, M., Asadi, A., King, D. S., Webber, T. D., Haneda, M., & Kieffer, T. J. (2009). Incretin release from gut is acutely enhanced by sugar but not by sweeteners in vivo. American Journal of Physiology, 296, 473-479.
  • Gardana, C., Scaglianti, M., & Simonetti, P. (2010). Evaluation of steviol and its glycosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high-performance liquid chromatography-mass spectrometry. Journal of Chromatography A, 1217, 1463-1470.
  • Geuns, J. M. (2003). Stevioside. Phytochemistry, 64, 913-921.
  • Goyal, S., Samsher, S., & Goyal, R. (2010). Stevia (Stevia rebaudiana) a bio-sweetener: A review. International Journal of Food Sciences and Nutrition, 61, 1-10.
  • Gupta, E., Purwar, S., Sandaram, S., & Gai, G. K. (2013). Nutritional and therapeutic values of Stevia rebaudiana: A review. Journal of Medicinal Plants Research, 7, 3343-3353.
  • Johnson, A. K. (2007). The sensory psychobiology of thirst and salt appetite. Medicine & Science in Sports & Exercise, 39, 1388-1400.
  • London, R. M., Snowdon, C. T., & Smithana, J. M. (1979). Early experience with sour and bitter solutions increases subsequent ingestion. Physiology and Behavior, 22, 1149-1155.
  • Prutkin, J., Fisher, E. M., Etter, L., Fast, K., Gardner, E., Lucchina, L. A., Snyder, D. J., Tie, K., Weiffenbach, J., & Bartoshuk, L. M. (2000). Genetic variation and inferences about perceived taste intensity in mice and men. Physiology and Behavior, 69, 161-173.
  • Reed, D. R., Li, S., Li, X., Huang, L., Tordoff, M. G., Starling-Roney, R., Taniguchi, K., West, D. B., Ohmen, J. D., & Beauchamp, G. K., et al. (2004). Polymorphisms in the taste receptor gene (Tas1r3) region are associated with saccharin preference in 30 mouse strains. Journal of Neurosciences, 24, 938-946.
  • Roberts, A., & Renwick, A. G. (2008). Comparative toxicokinetics and metabolism of rebaudioside A, stevioside, and steviol in rats. Food Chemical Toxicology, 46, 31-39.
  • Schiffman, S. S., Booth, B. J., Losee, M. L., Pecore, S. D., & Warwick, Z. S. (1995). Bitterness of sweeteners as a function of concentration. Brain Research Bulletin, 36, 505-513.
  • Sclafani, A., & Abrams, M. (1986). Rats show only a weak preference for the artificial sweetener aspartame. Physiology and Behavior, 37, 253-256.
  • Sclafani, A., Bahrani, M., Zukerman, S., & Ackroff, K. (2010). Stevia and saccharin preferences in rats and mice. Chemical Senses, 35, 433-443.
  • Sclafani, A., & Clare, R. A. (2004). Female rats show a bimodal preference response to the artifi cial sweetener sucralose. Chemical Senses, 29, 523-528.
  • Sclafani, A. (2006). Oral, post-oral and genetic interactions in sweet appetite. Physiology and Behavior, 89, 525-530.
  • Tennant, D. (2010). Potential intakes of steviol glycosides from use as a sweetener. Report prepared for the Union of European Beverages Associations (UNESDA).
  • Tordoff, M. G., Alarcon, L. K., & Lawler, M. P. (2008). Preferences of 14 rat strains for 17 taste compounds. Physiology and Behavior, 95, 308-332.
  • Valenstein, E. S. (1967). Selection of nutritive and nonnutritive solutions under different conditions of need. Journal of Comparative Physiology and Psychology, 63, 429-433.
  • Wagner, M. H. (1971). Comparative rodent preferences for artifi cial sweeteners. Journal of Comparative Physiology and Psychology, 75, 483-490.
  • Wheeler, A., Boileau, A. C., Winkler, P. C., Compton, J. C., Prakash, I., Jiang, X., Mandarino, D. A. (2008). Pharmacokinetics of rebaudioside A and stevioside after single oral doses in healthy men. Food Chemical Toxicology, 46, 54-60.
  • Yesmine, S., Connolly, K., Hill, N., Coulson, F. R., & Fenning, A. S. (2013). Electrophysiological, vasoactive, and gastromodulatory effects of stevia in healthy Wistar rats. Planta Medica, 79, 909-915.