Aislamiento de núcleos para citometría de flujo en plantas con alto contenido de compuestos mucilaginosos: un ejemplo en el género Viola L. (Violaceae)un ejemplo en el género Viola L. (Violaceae)

  1. Cires Rodríguez, Eduardo
  2. Cuesta Moliner, Candela
  3. Fernández Casado, María de los Angeles
  4. Nava Fernández, Herminio S.
  5. Vázquez, Víctor M.
  6. Fernández Prieto, José Antonio
Journal:
Anales del Jardín Botánico de Madrid

ISSN: 0211-1322

Year of publication: 2011

Volume: 68

Issue: 2

Pages: 139-154

Type: Article

DOI: 10.3989/AJBM.2273 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Anales del Jardín Botánico de Madrid

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Abstract

Cires, E., Cuesta, C., Fernández, M.A., Nava, H.S., Vázquez, V.M. & Fernández, J.A. 2011. Isolation of plant nuclei suitable for flow cytometry from species with extremely mucilaginous compounds: an example in the genus Viola L. (Violaceae). Anales.Iard. Bot. Madrid 68(2): 139-154. Flow cytometry analysis has been widely appljed in the determination of nuclear DNA content and ploidy level in many organisms. Despite being the most appropriate method for DNA content measurement, flow cytometry also presents some limitations. A fairly common, but little-studied problem is the effect on measurements of the presente of secondary metabolites. A good example is the genus Viola, which is composed of 525-600 species distributed worldwide. These species have proved to be problematic for flow cytometric analyses due to the release of extremely mucilaginous compounds into the nuclear suspension. In this work, the genome size of 13 species of Viola using flow cytometry are presented for the first time. Despite obtaining histograms with high coefficients of variation, we here pre-sent an optimized protocol to remove cytoplasmic compounds, particularly mucilaginous ones, from plant nudei that paye the way for its application to estimate the genome size of other species exhibiting similar problems. Statistical analyses revealed significant differences between sections Viola and Melanium, and within each section (P < 0.001). Furthermore, statistically significant differences were not detected among samples of the same species.

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